Entire Human Genome on a 1.5 cm chip (plus boobies!)

[SpeedRacer 1]

Check this out: This is an Affymetrix GeneChip. It has the complete human genome on it. It is attached in 25 basepair lengths to this little chip that is 1.5 cm on each side.

There are little ports on the back where you can inject your RNA samples. This technology can be used to find out which genes are being transcribed by a given set of cells.

So for example, you can stimulate some T-lymphocytes with something, isolate the RNA, label it with a fluorescent dye, and inject it onto the chip. The chip is analyzed with a laser beam, and the computer can tell which genes are being used by the cells by detecting where the fluorescent dye shows up on the chip. you can compare stimulated cells to unstimulated cells & find out exactly what that compound makes the cell do.

You can analyze over 47,000 different RNA transcripts at once.

(This is a used one. I took the pic with my cell phone camera to show you how small it is. The little glass square in the middle is the chip. edited to add: you can see the reflection of my cell phone in the glass.)


edited to add: the second pic entitled chloe9-88 is a NSFW, completely gratuitous photo of boobies. I edited it to add these boobies, because the first time I made this post without it, and the thread just sank like a rock.

Speed Racer
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[Squeak 17]

That is VERY VERY Cool
Nano Science is on oour door step

You are not now, nor will you ever be, good enough to not die in this sport (Sparky)
My Life ROCKS!
How's yours doing?

[freeflir29 0]

Nice boobies.

[kbordson 8]

WAY COOL!!! What do you do that you get to play with such neat toys!
Genetic sequencing on a chip, watch out for GATTACCA....

[CrazyIvan 0]

Yummmm...boobies.

__________________________________________
Blue Skies and May the Force be with you.

[freeflir29 0]

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WAY COOL!!! What do you do that you get to play with such neat toys!

Geez...............OBVIOUSLY he works at a titty bar!

[SpeedRacer 1]

A couple years ago someone came to where I work & gave a seminar about this. Then they showed the gene expression profiles of cells from several different human tissues.

What they found was that the gene expression profiles of the testicles were REMARKABLY similar to the expression profile from brain cells.

Draw your own conclusions.

Speed Racer
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[Slappie 9]

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Genetic sequencing on a chip, watch out for GATTACCA....

That is such a true statement. Even though Science Fiction has warned us of these things in the past. Some still hide under rocks and say it isn't so and could never happen. It's already happening. Clinics opening up claiming they can genderise your next pregnancy. "Want a boy? come see us we'll make it happen. Want a blonde female with large breasts when she grows up? Come see us we'll make it happen."

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Gattaca is a futuristic thriller that warns about the dangers of gene technology.. where it could lead if allowed to be developed and let to be the new form of discrimination: genoism replacing e.g. racism.

The Human Genome is made up of 4 strings of DNA: A, C, G & T. So GATTACCA is the string of DNA used to make the clone.

I don't want to sound paranoid but man that kinda shit spooks me.

"Find out just what any people will quietly submit to and you have found out the exact measure of injustice and wrong which will be imposed upon them."

[Keith 0]

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edited it to add these boobies, because the first time I made this post without it, and the thread just sank like a rock.

Thanks to the addition, this thread is sure to explode like a virgin during his first orgasm.

Keith

Don't Fuck with me Keith - J. Mandeville

[ChrisL 2]

I got yer genome...right here!

__

My mighty steed

[JohnRich 4]

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You can analyze over 47,000 different RNA transcripts at once.

Yeah, but I bet you can't get Chloe's (the NSFW photo girl) phone number even once.

[SpeedRacer 1]

aw crap. I was hoping there were enough people on here who were interested in scientific progress, that this would stay up on top for a while.

Turns out, over half the respondents were only interested in the boobies.

I am so disappointed.

Oh look, its back up at the top again!

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[Slappie 9]

Give me some examples as to how your research is helping the human race? Other then altering genes to do your bidding. Then creating a super bug that's going to wipe out the entire human race.

"Find out just what any people will quietly submit to and you have found out the exact measure of injustice and wrong which will be imposed upon them."

[Dougiefresh 0]

Those things are sweet. I wanna work for Affymetrix. But they won't let me.

Those things are sweet. I wanna be a photographer of naked ladies. But they won't let me.

Human beings, who are almost unique in having the ability to learn from the experience of others, are also remarkable for their apparent disinclination to do so. --Douglas Adams

[kbordson 8]

You put the boobies in your attachment.. but not on the title list.....

That was one of your mistakes.

The other mistake was not telling more about the genetics thing.....

edit... sorry, you did have boobies on the thead title... sorry. I was just so excited about your genome. Who cares about boobies?

[SpeedRacer 1]

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You put the boobies in your attachment.. but not on the title list.....

?? But it is in the title!

Anyway, I don't personally use these chips. There was a woman in the lab downstairs from mine who was analyzing some samples of a colleague of mine, & I watched her do it.

As to how my research benefits mankind? (Answering the heavily-armed groundhog's question here) I do research on HIV. We're looking at the way HIV becomes resistant to certain drugs. We have sequenced the protease gene from HIV taken from patients un-responsive to HIV protease inhibitors. Then we induce these mutations in HIV one by one & grow them in cells in the presence of different Protease inhibitors, to see which mutation causes what sort of resistance.

There is a database containing a list of known mutations & a chart showing which ones cause resistance to which drug. What this means is that when a doctor is treating a new patient with HIV, he can take a sample, get it sequenced, and access the database to find out which drugs to use and which drugs won't work.

so anyway, that is how my research benefits mankind.

(I'm creating the genetically-altered superbug to take over the world in my spare time. )

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[kbordson 8]

I editted my reply... dicha read that? Nooooo.... you just focus on the negative. Look to the positive - the butterflies, the sun shining so bright and pretty... the - ohhh shiny.

besides, I bumped your thead back up again.... and said that I was interested in your genome. In geek, that means something crude - I think.

Karen

[SpeedRacer 1]

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Genetic sequencing on a chip, watch out for GATTACCA

well the chip isn't used for sequencing. It's used for finding out which gene is being expressed, not the gene's sequence.

When I perform mutagenesis on a plasmid, I always sequence the result. But you don't use a chip. You first perform an enzymatic reaction on the plasmid & then you run it through a big capillary sequencer thingy.

You get to use a lotta neat tools when you clone.

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[kallend 2,174]

I have my genome in the nucleus of every cell, which in turn is so tiny that you need a microscope to see it.

Edited to add "thanks for the boobies"

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The only sure way to survive a canopy collision is not to have one.