Making your own TSB plates

[ladyskydiver 0]

For those who have made their own TSB plates, ideas/thoughts on why the agar (that's was partially gelled in the plate on the table and then put into a 35C incubator) would turn into liquid instead of finishing solidifying?

Life is short! Break the rules! Forgive quickly! Kiss slowly! Love truly, Laugh uncontrollably. And never regret anything that made you smile.

[jumper03 0]

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For those who have made their own TSB plates, ideas/thoughts on why the agar (that's was partially gelled in the plate on the table and then put into a 35C incubator) would turn into liquid instead of finishing solidifying?

I'd rather poke forks in my eyes....

Scars remind us that the past is real

[ladyskydiver 0]

Gotta love microbiology lab and growing cultures.

Life is short! Break the rules! Forgive quickly! Kiss slowly! Love truly, Laugh uncontrollably. And never regret anything that made you smile.

[jumper03 0]

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Gotta love microbiology lab and growing cultures.

next time you're on msn, I'll tell about an alternative growth medium my student and I are developing for zero-g long duration space flight

beats agar hands down

Scars remind us that the past is real

[ladyskydiver 0]

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Gotta love microbiology lab and growing cultures.

next time you're on msn, I'll tell about an alternative growth medium my student and I are developing for zero-g long duration space flight

beats agar hands down

Unfortunately, we don't get a choice in our agar. Whatever the experiment calls for is what we have to use.

Life is short! Break the rules! Forgive quickly! Kiss slowly! Love truly, Laugh uncontrollably. And never regret anything that made you smile.

[JohnnyD 0]

Check the temperature of the incubator. Also, if its overloaded, it could throw the control characteristics off.

[Squeak 17]

Cora make sure you are using enough of the Agar powder when you mix it. also what nutirents are you using in the mix, if they are in liquid form they need to ne considiered in the total volume

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[ladyskydiver 0]

The mix had been made for us so I'm not sure what Agar powder and nutrients were used and was sitting in a 60C water bath. We had already made 1/2 a plate and allowed it to gel for a week. When the issue came in was when we took the mix that was in the water bath, inoculated our Escherichia coli bacteriophage culture with the liquid agar (it was cool to the inside of the wrist before we inoculated our culture) and then poured it onto the already gelled plate. It started gelling on the counter at room temp and then when put into the incubator, eventually liquified.

The reason that I'm mystified is that there were 80 plates made by the class and put into the incubator, and the incubator could have handled even more if the plates had been stacked. Ours liquified and a few others had no cultures grow while some had cultures grow.

We've tossed around the idea that somehow our Agar was contaminated or we ended up with a bad batch of it, but I'm not satisfied with that as there seems to be other possibilities.

Life is short! Break the rules! Forgive quickly! Kiss slowly! Love truly, Laugh uncontrollably. And never regret anything that made you smile.

[Remster 30]

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I'll tell about an alternative growth medium my student and I are developing

Is that one of them more the profesionnal, less then romantic situations?

Remster